麦冬多糖脂质体增强雏鸡免疫功能的分析

doi:10.11843/j.issn.0366-6964.2015.07.023

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (7): 1246-1252.doi: 10.11843/j.issn.0366-6964.2015.07.023

麦冬多糖脂质体增强雏鸡免疫功能的分析

范云鹏，马琳，张为民，宋晓平^*，郭超，侯伟峰，童德文^*

（西北农林科技大学动物医学院，杨凌 712100）

收稿日期:2014-11-07 出版日期:2015-07-23 发布日期:2015-07-23
通讯作者: 童德文，教授，E-mail：dwtong@nwsuaf.edu.cn；宋晓平，教授，E-mail：sxpxbnl@163.com
作者简介:范云鹏（1983-），男，山东烟台人，讲师，博士，主要从事兽医中药学与中药药理学研究，E-mail：ypfan@nwsuaf.edu.cn
基金资助:
国家自然科学基金项目（31402240）；中央高校基本科研业务费（2452015037）；中国博士后科学基金项目（2014M550516）

Analysis of Ophiopogon Polysaccharide Liposome on Enhancing the Immune Function in Chickens

FAN Yun-peng，MA Lin，ZHANG Wei-min，SONG Xiao-ping^*，GUO Chao，HOU Wei-feng，TONG De-wen^*

(College of Veterinary Medicine，Northwest A&F University，Yangling 712100，China)

Received:2014-11-07 Online:2015-07-23 Published:2015-07-23

摘要/Abstract

摘要：

为探讨麦冬多糖脂质体（OPL）的增强免疫活性，进行以下试验。体外试验，采用MTT法测定OPL对雏鸡脾中T、B淋巴细胞增殖的影响，并以麦冬多糖（OP）作为对照；体内试验，400只11日龄雏鸡随机均分为8组，先用环磷酰胺进行免疫抑制造模，14日龄时用新城疫疫苗进行免疫，在免疫的同时注射不同剂量的OPL，然后测定免疫后不同时间点OPL对外周血淋巴细胞增殖、抗体效价和细胞因子含量的影响。结果显示，在体外，OPL在7.813~125 μg•mL^-1时无论是单独作用还是协同PHA、LPS作用均能显著促进鸡脾淋巴细胞的增殖，且效果显著优于OP（P<0.05）；在体内，OPL高、中剂量能够显著促进淋巴细胞增殖，增强抗体效价，提高细胞因子IL-2和IFN-γ的含量，且效果显著优于OP（P<0.05）。结果表明，OPL能够显著提高OP的增强免疫活性，有望开发成新型的麦冬多糖制剂。

Abstract:

In order to investigate the immune-enhancing activity of ophiopogon polysaccharide liposome (OPL)，two experiments were carried out.In vitro，the effects of OPL on splenocyte proliferation of chicken were determined by MTT method，and ophiopogon polysaccharide (OP) was used as control.In vivo，four hundred 11-day-old chickens were randomly assigned into 8 groups，and the chickens were injected with cyclophosphamide to build the immunosuppression model.At 14-day-old，the chickens were vaccinated with Newcastle disease vaccine.At the same time of the first vaccination，the chickens were injected respectively with OPL at different dosages.At various time points after the first vaccination，the peripheral lymphocyte proliferation，serum antibody titer and the contents of cytokines were measured.The results showed that OPL could significantly promote splenocyte proliferation singly or synergistically with PHA and LPS at 7.813-125 μg•mL^-1 in vitro，and the efficacy was superior to OP (P<0.05)；In vivo，OPL at high and medium doses could significantly promote lymphocyte proliferation，enhance antibody titer and improve the contents of IL-2 and IFN-γ compared with OP at most time points (P<0.05).These results indicated that OPL could significantly improve the immune-enhancing activity of OP，OPL would be expected to exploit into a new-type preparation of OP.

中图分类号:

S853.74

范云鹏，马琳，张为民，宋晓平，郭超，侯伟峰，童德文. 麦冬多糖脂质体增强雏鸡免疫功能的分析[J]. 畜牧兽医学报, 2015, 46(7): 1246-1252.

FAN Yun-peng，MA Lin，ZHANG Wei-min，SONG Xiao-ping，GUO Chao，HOU Wei-feng，TONG De-wen. Analysis of Ophiopogon Polysaccharide Liposome on Enhancing the Immune Function in Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1246-1252.

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麦冬多糖脂质体增强雏鸡免疫功能的分析

Analysis of Ophiopogon Polysaccharide Liposome on Enhancing the Immune Function in Chickens

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